Biomedical Press, Amsterdam -- Printed in The Netherlands RESTRICTION MAP, PARTIAL CLONING AND LOCALIZATION OF 9S AND 12S KINETOPLAST RNA GENES ON THE MAXICIRCLE COMPONENT OF ~ KINETOPLAST DNA OF Leishmania tarentolae
نویسندگان
چکیده
We have constructed a restriction map of the maxicircle component of the kinetoplast DNA of Leishmania tarentolae for the enzymes EcoRI, BamHI, HaeIII, HpaII, SalI, BglII and HindIII. The 9 and 12S kinetoplast RNAs were localized on this map. Two fragments of this maxicircle molecule were cloned in the bacterial plasmid, pBR322, including a 4 .4 .106 dalton EcoRI/BamHI fragment which contains the 9 and 12S RNA genes.
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